VISCOUS LIQUID CLASS DEVELOPMENT

Liquid handling for viscous liquids often requires an expert touch to get it done effectively. On a Tecan Freedom Evo liquid handling robot with disposable tips, the following parameters are a good place to start:

1. Aspiration rate: 10% of Water Free Dispense default class

2. Delay: 10s 

3. Tip retract: 5 mm/s

4. Wet contact, as far into the liquid level as allowed.

5. Dispense rate: 10% of Water Free Dispense default class

6. Delay: 10s

7. Tip retract: as slow as possible while still meeting your timing goals.

There are many more qualities to consider than just the “viscosity” of the liquid, and these qualities will change how you pipette, whether: positive displacement, blow outs, tip touch, etc.

To expedite method development and assay optimization, get someone who develops liquid classes using your samples and reagents, not just water tests, and someone who does it professionally.

Contact us today to learn more about how we've helped labs like yours achieve goals faster and cheaper.